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In vivo tumouricidal effects of LAD-1 monoclonal antibody on murine RL-male-1 lymphoma mediated by enhanced phagocytosis.
- Source :
-
Clinical & Experimental Immunology . Jul2005, Vol. 141 Issue 1, p54-61. 8p. - Publication Year :
- 2005
-
Abstract
- We have reported previously that the LAD-4 monoclonal antibody (mAb) directed against a fibronectin receptor (FNR) on RL-male-1 T lymphoma cells in BALB/c mice partially inhibited their migration to the liver. In the present study, we examined the mechanism by which another anti-FNR mAb, LAD-1, exerts its antitumourigenic effects. Administration of LAD-1 significantly prolonged survival of BALB/c mice challenged previously with RL-male-1 cells. LAD-1 enhanced phagocytosis of RL-male-1 cells by hepatic macrophages and clodronate-mediated macrophage depletion abrogated the antitumour activity of LAD-1. In vitro experiments revealed that a pan-caspase inhibitor, zVAD-fmk, did not affect the ability of LAD-1 to inhibit the proliferation of RL-male-1 cells. These data suggest that the antitumour effects of LAD-1 may be dependent on stimulation of tumour cell phagocytosis and are apoptosis-independent. Thus, LAD-1-induced phagocytosis of lymphoma cells by hepatic macrophages in mice may, at least in part, be responsible for the prolonged survival of the mice. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANTIGEN-antibody reactions
*IMMUNE response
*CELLS
*MICE
*PHAGOCYTOSIS
*ENDOCYTOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 141
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17298131
- Full Text :
- https://doi.org/10.1111/j.1365-2249.2005.02809.x