Biochar facilitates biofuel production through batch single-stage and two-stage digestion of grass silage and cattle slurry.

Grass silage and cattle slurry are the most abundant feedstocks for biogas in an Irish context. This work describes how to optimize their biomethane yield. Hydrolysis and biomethane yields from co-digestion of grass silage and slurry were enhanced in a biochar-supplemented two-stage digestion process. Biochar addition at the optimal dosage of 10 g/L in batch two-stage digesters led to the highest methane yield of 253 L per kilogram (kg) volatile solid (VS), which was 24% higher than that from two-stage digesters without biochar supplementation. For a batch single-stage digester, the biomethane yields were lower than the two-stage but 10 g/L biochar addition led to an increase to 218 L/kg VS as compared to 198 L/kg VS for the group without biochar supplementation. Volatile fatty acid accumulation increased with the enhancement of biomethane production in the biochar-supplemented two-stage reactors. The addition of biochar in single-stage and two-stage anaerobic digesters presented positive effects on the electrochemical properties of the digester contents, reflected by the increased charge storage capacity, reduced solution resistance and improved digester content conductivity. The methane yields in two-stage anaerobic digestion were shown to be strongly correlated (R2 = 0.92) with the concentration of extracellular polymeric substances. This evidenced that the interlaced conductive network established by extracellular polymeric substances and biochar was essential for direct interspecies electron transfer and biomethane production. This study demonstrated that biochar-supplemented two-stage anaerobic digestion is an optimal route for energy recovery from grass silage and cattle slurry. [Display omitted] • Co-digestion of on-farm feedstock was enhanced in a two-stage process with biochar. • Biochar at 10 g/L in two-stage digestion maximized CH 4 yield at 253 L/kg VS. • Biochar at 10 g/L in single-stage digestion achieved a CH 4 yield of 218 L/kg VS. • Biochar improved sludge conductivity to facilitate interspecies electron transfer. • CH 4 yield highly correlated with extracellular polymeric substances concentration. [ABSTRACT FROM AUTHOR]