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Sperm-borne lncRNA loc100847420 improves development of early bovine embryos.
- Source :
-
Animal Reproduction Science . Oct2023, Vol. 257, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Long non-coding RNAs (lncRNAs) act as competing endogenous RNAs (ceRNAs) that play a significant role in bovine embryo development; but the influence of sperm-borne lncRNA on the preimplantation development of bovine embryos has not been reported in detail. In this study, we aimed to clarify how sperm-borne lncRNAs can act to regulate early development of bovine embryos. Utilizing high-throughput sequencing technology and quantitative real-time PCR (qPCR), we found that the lncRNA, loc100847420, was highly enriched in bovine sperm and was carried into the oocyte during fertilization. Introduction of wild-type loc100847420 had no effect on cleavage rate of parthenogenetic embryos compared with injection of mutant loc100847420 (70.58 ± 2.85% vs 70.46 ± 1.98%, p > 0.05), but significantly improved the blastocyst rate (33.67 ± 2.40% vs 28.35 ± 3.06%, p < 0.05), total numbers of cells (p < 0.05), numbers of inner cell mass (ICM) cells (p < 0.05) and numbers of trophoblast (TE) cells (p < 0.05). In summary, the sperm-borne lncRNA, loc100847420, can improve the developmental potential of early bovine embryos. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SPERMATOZOA
*LINCRNA
*EMBRYOS
*BOS
*NUCLEOTIDE sequencing
*OVUM
Subjects
Details
- Language :
- English
- ISSN :
- 03784320
- Volume :
- 257
- Database :
- Academic Search Index
- Journal :
- Animal Reproduction Science
- Publication Type :
- Academic Journal
- Accession number :
- 172870860
- Full Text :
- https://doi.org/10.1016/j.anireprosci.2023.107333