Confirmation of a five‐way herbicide‐resistant Amaranthus tuberculatus population in North Carolina.

Amaranthus tuberculatus (waterhemp) is a pervasive weed of the Mid‐west and ‐south United States and is not native to North Carolina but infestations in crop fields have been reported recently. Amaranthus tuberculatus has evolved resistance to seven herbicide groups and multiple herbicide‐resistant populations are common where the species is native. The reported A. tuberculatus infestations in North Carolina have not been controlled with herbicides but no formal herbicide resistance characterisation has been conducted to date. Glasshouse dose–response experiments were conducted to determine the susceptibility of a population collected from Surry County, North Carolina to commonly applied postemergence herbicides compared to a herbicide‐susceptible population collected from Story County, Iowa. The Surry County population survived labelled rates of imazethapyr, atrazine, glyphosate, fomesafen, and mesotrione; the Story County population was controlled with these herbicides. Further, 2,4‐D, dicamba, and glufosinate effectively controlled the Surry and Story County populations. Molecular sequencing assays were subsequently conducted to determine if altered target sites facilitated resistance in the acetolactate synthase (ALS), 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS), photosystem II (psbA), and protoporphyrinogen oxidase (PPX2) genes. The Surry County population carried a Trp574Leu and ∆Gly210 mutations in the ALS and PPX2 gene, respectively. No mutations that would confer resistance were found in the EPSPS or psbA gene for either population. The results of both experiments provide evidence that a five‐way herbicide‐resistant A. tuberculatus population has encroached North Carolina. More research is needed to determine the mechanisms of resistance to atrazine, glyphosate, and mesotrione. [ABSTRACT FROM AUTHOR]