血清25-(OH)D水平及VDR基因多态性与类风湿性关节炎骨侵蚀的关系探讨.

Objective To explore the relationship among serum 25-hydroxyvitamin D [25-(OH)D], vitamin D receptor (VDR) gene polymorphism, and bone erosion in patients with rheumatoid arthritis. Methods Ninety-four patients with rheumatoid arthritis were divided into study group and 85 healthy persons were divided into health group. The serum 25- (OH)D levels were detected in the 2 groups. The genomic DNA was extracted and the VDR gene was detected using MassARRAY molecular weight array technology platform. The relationship between serum 25-(OH)D level and VDR gene polymorphism and bone erosion in rheumatoid arthritis was analyzed. Results The serum 25-(OH)D level in the study group was significantly lower than that in the healthy group (P<0.05), and it was lower in the patients with bone erosion than in the patients without bone erosion (P<0.05). The frequencies of genotype FF, Ff, and allele F of FokI locus and genotype AA, and allele A of ApaI locus in the study group were significantly higher than those in the healthy group (P<0.05). The frequencies of genotype ff and aa in the study group were significantly lower than those in the healthy group (P<0.05). Serum 25-(OH) D less than 30 ng/mL, FokI genotype FF and Ff, and ApaI genotype AA and Aa were risk factors for rheumatoid arthritis (P < 0.05). Serum 25-(OH)D less than 20 ng/mL was a risk factor for bone erosion in rheumatoid arthritis (P<0.05). Conclusion Lack of serum 25-(OH)D increases the risk of bone erosion in patients with rheumatoid arthritis. The allele F of FokI and the allele of ApaI of VDR gene increase the risk of rheumatoid arthritis, but VDR gene is not associated with bone erosion. [ABSTRACT FROM AUTHOR]