葡萄VvWRKY70 基因生物信息学及表达特性分析.

WRKY transcription factors(TFs), as plant-specific transcription factors, play significant roles in various biological and abiotic stress. In this study, based on RNA-seq analyses, many differentially expressed grapes WRKY genes with unknown function were found during the process of SO2 fumigation combined with low temperature preservation. Through amino acid sequence homology comparison, VvWRKY70 which had the highest similarity to the amino acid sequence of AtWRKY70 was selected for bioinformatics and expression characteristics analysis. The results of bioinformatics analysis showed that the promoter region of VvWRKY70 gene contained W-box element and the response elements to the hormones such as methyl jasmonate and salicylic acid. VvWRKY70 encoding protein was composed of 322 amino acids with a molecular weight about 36.57 ku and hydrophilic, rich in serine/threonine. The secondary structure was mainly composed of random coil and α -helix. The N-terminal contained a highly conserved WRKY domain, and the C-terminal was a C2HC zinc finger structure, belonging to the third WRKY subfamily. Predictions also showed that VvWRKY70 protein was mainly located in the nucleus and conserved in evolution. Analysis of qRT-PCR showed that the VvWRKY70 expressed in Muscat root, stem, leaf, bud, and fruit. The expression of VvWRKY70 in fruit was up-regulated by SO2 and Botrytis cinerea, and down-regulated by low temperature. These results revealed that VvWRKY70 was probably involved in regulation of the process of plant physiology and fruit development, played a role in the process of SO2 preservation of fruits after harvest, and regulated the biotic and abiotic stress responses during fruit storage. [ABSTRACT FROM AUTHOR]