Copper enhanced arsenic-accumulation in As-hyperaccumulator Pteris vittata by upregulating its gene expression for As uptake, translocation, and sequestration.

In contaminated soils, arsenic (As) often co-exists with copper (Cu). However, its effects on As accumulation and the related mechanisms in As-hyperaccumulator Pteris vittata remain unclear. In this study, P. vittata plants were exposed to 50 µM As and/or 50 µM Cu under hydroponics to investigate the effects of Cu on plant growth and As accumulation, as well as gene expression related to arsenic uptake (P transporters), reduction (arsenate reductases), and translocation and sequestration (arsenite antiporters). After 14 d of growth and compared to the As treatment, the As concentration in P. vittata fronds increased by 1.4-times from 793 to 1131 mg·kg−1 and its biomass increased by 1.2-fold from 18.0 to 21.1 g·plant−1 in the As+Cu treatment. Copper-enhanced As accumulation was probably due to upregulated gene expressions related to As-metabolisms including As uptake (1.9-fold in P transporter PvPht1;3), translocation (2.1–2.4 fold in arsenite antiporters PvACR3/3;2) and sequestration (1.5–2.0 fold in arsenite antiporters PvACR3;1/3;3). Our results suggest that moderate amount of Cu can help to increase the As accumulation efficiency in P. vittata , which has implication in its application in phytoremedation in As and Cu co-contaminated soils. [Display omitted] • 50 µM Cu enhanced plant growth and As accumulation in P. vittata. • P. vittata maintains a normal Cu level in fronds by concentrating most Cu in roots. • Cu promoted AsV uptake by upregulating P transporter PvPht1;3. • Cu upregulated AsIII antiporters PvACR3-3;3 for As translocation and sequestration. [ABSTRACT FROM AUTHOR]