The Value of Concomitant Measurement of miR 323b-5p and miR 486-5p with Ankle Brachial Index in Diagnosis of Peripheral Arterial Disease.

Background: Peripheral artery disease (PAD) is an advanced atherosclerotic disease-causing augmented risks of hospitalization and death. So, several studies tried to detect novel biomarkers for PAD diagnosis. The study is aimed to explore the role of plasma miRNA 323b-5p and miRNA 486-5p levels to diagnose PAD. Methods: The research was performed in Medical Biochemistry & Cardiology Departments - Zagazig Faculty of Medicine. It included 100 volunteers categorized into 2 groups; Group (1): included 50 healthy volunteers served as control group with ankle brachial index (ABI) > 0.9. Group (2): included 50 PAD patients with ABI < 0.9. Plasma 323b-5p and miR 486-5p expression level were measured via real time PCR. Color Doppler was performed to calculate ABI, which was correlated with miRNA levels. Results: There was a highly significant difference between groups as regards miR 323b-5p. Furthermore, miR 486-5p expression level showed significant variance between groups. Regarding miRNA 323b-5p expression level, cutoff value was 2.55, sensitivity was 89.3% and specificity was 72.7%. Concerning miR 486-5p expression level, cutoff value was 1.92, sensitivity was 82.1% and specificity was 81.8%. As regards combined miR 323b-5p and miR 486-5p ROC curve, it was 96.4% sensitivity and 95.5% specificity. Each miRNA showed a negative correlation with ABI. Conclusion: miRNA 323B-5P and miRNA 486-5P can be a novel and potent biomarker for PAD diagnosis, particularly for risky patients. [ABSTRACT FROM AUTHOR]