α-月桂酸单甘油酯和 吡咯喹啉醌二钠对蛋鸡肝脏脂质代谢的影响.

The aim of the study was to investigate the effect of α-GML and PQQ·Na2 and their combination on liver lipid metabolism in late laying Hyline brown laying hens. A total of 384 fifty-two-week-old Hyline brown laying hens with similar body weight were randomly divided into four groups with four replicates per group and 24 hens per replicate. Hens in Con group were fed with basal diet. The GML group, PQQ group and GML+PQQ group were supplemented with 800 mg/kg α-GML, 0.16 mg/kg PQQ·Na2, 800 mg/kg α-GML + 0.16 mg/kg PQQ·Na2, respectively. The pre-test lasted for 2 w, and the formal test lasted for 8 w. The results showed that compared with control group, serum AST and ALT activity, TC and TG content, liver TC and TG content, liver fat percentage and abdominal fat percentage of laying hens in experimental groups were significantly or extremely decreased (P<0.05 or P<0.01). The mRNA expression levels of ATGL and AMPKα1 in liver of hens in GML group were significantly increased (P<0.05), while the expression levels of ACC, FASN, SCD-1 and SREBP-1c were extremely or significantly decreased (P<0.01 or P<0.05). The expression levels of FASN, SCD-1 and CPT-1 in liver of hens in PQQ group were extremely or significantly decreased (P<0.01 or P<0.05). The expression level of ATGL in liver of hens in GML + PQQ group was significantly increased (P<0.05), while the expression levels of ACC, FASN, SCD-1, SREBP-1c and PPARγ were significantly or extremely decreased (P<0.05 or P<0.01). The study indicates that dietary α -GML and PQQ·Na2 and their combination can regulate liver lipid metabolism and reduce liver lipid deposition of laying hens, and the combination of the two additives has a more significant effect on reducing liver lipid deposition of laying hens. [ABSTRACT FROM AUTHOR]