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Development of soft X-ray ptychography and fluorescence microscopy system using total-reflection wolter mirror and application to measurement of drug-treated mammalian cells.

Authors :
Takeo, Yoko
Sakurai, Kai
Furuya, Noboru
Yoshinaga, Kyota
Shimamura, Takenori
Egawa, Satoru
Kiuchi, Hisao
Mimura, Hidekazu
Ohashi, Haruhiko
Harada, Yoshihisa
Shimura, Mari
Kimura, Takashi
Source :
Journal of Electron Spectroscopy & Related Phenomena. Aug2023, Vol. 267, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

We developed a soft X-ray microscopic imaging system, CARROT, that combines ptychography and fluorescence X-ray microscopy using a total-reflection Wolter mirror illumination system. The system offers advantages such as achromaticity and long working distances, enabling seamless application to a wide wavelength range. We demonstrated high-resolution imaging of cellular structures and sub-micrometer-scale changes induced by drug administration in mammalian cells. We further showcased the capability to seamlessly switch from ptychography measurements to X-ray fluorescence measurements at different wavelengths to map elemental distributions within cells. Our findings suggest that this system can be used for investigating the correlation between intracellular structures and elemental distribution, as well as for multimodal measurements of visible light fluorescence, Raman scattering, and soft X-rays to provide valuable insights into understanding intracellular dynamics. • New imaging system combines soft X-ray ptychography & fluorescence microscopy. • High-resolution images of drug-treated mammalian cells reveal sub-micrometer changes. • Investigation of the correlation between intracellular structures and elemental distribution. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03682048
Volume :
267
Database :
Academic Search Index
Journal :
Journal of Electron Spectroscopy & Related Phenomena
Publication Type :
Academic Journal
Accession number :
171848656
Full Text :
https://doi.org/10.1016/j.elspec.2023.147380