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A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs

Authors :
Decaro, Nicola
Elia, Gabriella
Martella, Vito
Desario, Costantina
Campolo, Marco
Trani, Livia Di
Tarsitano, Elvira
Tempesta, Maria
Buonavoglia, Canio
Source :
Veterinary Microbiology. Jan2005, Vol. 105 Issue 1, p19-28. 10p.
Publication Year :
2005

Abstract

Abstract: We describe a rapid, sensitive and reproducible real-time PCR assay for detecting and quantifying canine parvovirus type 2 (CPV-2) DNA in the feces of dogs with diarrhea. An exogenous internal control was added to control the assay performance from extraction to amplification. The method was demonstrated to be highly specific and sensitive, allowing a precise CPV-2 DNA quantitation over a range of eight orders of magnitude (from 102 to 109 copies of standard DNA). The reproducibility of the CPV-2 real-time PCR assay was assessed by calculating the coefficients of variation (CV) intra-assay and inter-assay for samples containing amounts of CPV-2 DNA spanning the whole range of the real-time PCR standard curve. Then, fecal specimens from diarrheic dogs were analyzed by hemagglutination (HA), conventional PCR and real-time amplification. Comparison between these different techniques revealed that real-time PCR is more sensitive than HA and conventional gel-based PCR, allowing to detect low viral titers of CPV-2 in infected dogs. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
03781135
Volume :
105
Issue :
1
Database :
Academic Search Index
Journal :
Veterinary Microbiology
Publication Type :
Academic Journal
Accession number :
17152480
Full Text :
https://doi.org/10.1016/j.vetmic.2004.09.018