Study on the relationship between the expression of SLIT3 and SPARCL1 protein and the recurrence after radical operation in cervical cancer.

Objective To investigate the relationship between the expression of SLIT3 and SPARCL1 protein and the recurrence of cervical cancer after radical operation. Methods From October 2019 to April 2021, 107 patients who underwent radical surgery for cervical cancer in the hospital were selected as the study subjects, and 30 patients with benign uterine lesions were selected as the control group ; The patients were followed up for 1 year and were divided into relapse group (n = 25) and non relapse group (n = 82) according to the follow-up results. The expression levels of SLIT3 and SPARCL1 mRNA were detected by real-time fluorescence quantitative analysis (qPCR), and the expression levels of SLIT3 and SPARCL1 protein were detected by immunohistochemistry. The correlation between the expression of SLIT3 and SPARCL1 protein and clinicopathological characteristics was analyzed. Cox model was used to analyze the risk factors of cervical cancer recurrence after radical operation. Results The expression of SLIT3 and SPARCL1 mRNA in cervical cancer group was significantly lower than that in control group (P < 0.05) ; The levels of SLIT3 and SPARCL1 mRNA in cervical cancer tissues of patients with recurrence were significantly lower than those of patients without recurrence (P < 0.05).Among 107 patients with radical operation, 25 patients recurred, the recurrence rate was 23.36%. The postoperative recurrence rate of patients with low expression of SLIT3 (31.15%) was significantly higher than that of patients with high expression of SLIT3 (13.04%). The postoperative recurrence rate of patients with low expression of SPARCL1 (32.31%) was significantly higher than that of patients with high expression of sparcli (9.52%). The difference was statistically significant (P < 0.05). The expression of SLIT3 and SPARCL1 in cervical cancer was correlated with the degree of differentiation, clinical stage, lymph node metastasis and depth of stromal invasion (P < 0.05), but not with age, pathological type and maximum diameter of tumor (P > 0.05 ). Cox univariate analysis showed that the degree of differentiation, clinical stage, lymph node metastasis, stromal invasion depth, SLIT3 protein expression and SPARCL1 protein expression were correlated with the recurrence of cervical cancer patients after radical surgery (P < 0.05 ) ; Cox multivariate analysis showed that moderate/high differentiation, stage IIA, lymph node metastasis, stromal invasion depth > 50%, low expression of SLIT3 protein and SPARCL1 protein were independent risk factors for recurrence of cervical cancer patients after radical surgery (P < 0.05). Conclusion the expression of SLIT3 and SPARCL1 in cervical cancer tissue is lower than that in normal cervical tissue. The low expression of SLIT3 and SPARCL1 protein is an independent risk factor for the recurrence of cervical cancer after radical operation, and has certain clinical significance in predicting the recurrence of cervical cancer after operation. [ABSTRACT FROM AUTHOR]