结直肠癌组织Tregs占比和Foxp3, IL-10, TGF-β1 mRNA表达变化及其意义.

Objective To explore the proportion of regulatory T cells (Tregs) in colorectal cancer (CRC) tissues and the expression of Foxp3, interleukin 10 (IL-10), and transforming growth factor β1 (TGF-β1 ), and to analyze the relationships between the proportion of Tregs and the clinicopathological parameters of CRC. Methods Twenty-four CRC tissues and 24 normal colon tissues were selected from surgical resection. Flow cytometry was used to detect the proportion of Tregs, and real-time quantitative PCR was used to detect the expression levels of Foxp3, IL-10 and TGF-β1 mRNA in these two groups. Spearman correlation analysis was used to analyze the relationships between the proportion of Tregs and the clinicopathological parameters of CRC. Results Compared with the normal colon tissues, the proportion of Tregs in CRC tissues increased (P<0. 05) . Compared with the normal colon tissues, the relative expression of Foxp3, IL-10 and TGF-β1 mRNA in CRC tissue increased (all P<0. 05) . Tregs proportion was correlated with the stage of CRC (P<0. 05) . Conclusions The proportion of Tregs in CRC tissues increased, and the expression of Foxp3, IL-10 and TGF-β1 increased. The proportion of Tregs was related to the tumor stage of patients, and Tregs played an immune escape role probably by regulating Foxp3, IL-10 and TGF-β1. [ABSTRACT FROM AUTHOR]