High sensitive detection of methamphetamine by high-performance aptasensing platform based on nickel oxide nanoparticles anchored on mxene.

[Display omitted] • MXene has been utilized as the unique modifier for sensing interface fabrication. • NiONPs layer has been electrodeposited on MXene/GCE surface to link to Apt. • Apt was tightly attached to NiONPs/MXene/GCE surface to provide MAMP aptasensor. • The designed aptasensor displayed a wide LDR of 1 pM to 50 mM for MAMP detection. • The obtained LOD (333.3 fM) is the lowest value of all reported MAMP sensors. The concerns about the everyday abuse of narcotic materials, as a great alarm for society, have boosted many demands for sensitive and selective tracing of opiates in human biofluids. Herein, by taking advantage of nickel oxide nanoparticles (NiONPs) and MXene (NiONPs/MXene), an efficient sensing platform has been synthesized. The NiONPs/MXene nanocomposite increased the surface area and embedded many sites to load more aptamer (Apt) molecules as the bioreceptors of methamphetamine (MAMP) on the surface in the providing of the high-performance aptasensor. MAMP was measured by the aptasensor under a wide linear dynamic range from 1 pM to 50 mM with a very low limit of detection (LOD) of 333.3 fM within 40 min by consuming very small quantities of samples (10 µL) for each analysis. The highly selective and sensitive aptasensor was able to satisfactory measure MAMP in human serum, saliva and urine samples. The good repeatability and stability of the aptasensor revealed that the applied strategy may be a promising alternative for MAMP assay in the biofluids and street samples that is highly required as a non-invasive method for legal and clinical organizations. [ABSTRACT FROM AUTHOR]