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Conformational change in an engineered biliverdin-binding cyanobacteriochrome during the photoconversion process.
- Source :
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Archives of Biochemistry & Biophysics . Sep2023, Vol. 745, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- Cyanobacteriochromes (CBCRs) derived from cyanobacteria are linear-tetrapyrrole-binding photoreceptors related to the canonical red/far-red reversible phytochrome photoreceptors. CBCRs contain chromophore-binding cGMP-specific phosphodiesterase/adenylate cyclase/FhlA (GAF) domains that are highly diverse in their primary sequences and are categorized into many subfamilies. Among this repertoire, the biliverdin (BV)-binding CBCR GAF domains receive considerable attention for their in vivo optogenetic and bioimaging applications because BV is a mammalian intrinsic chromophore and can absorb far-red light that penetrates deep into the mammalian body. The typical BV-binding CBCR GAF domain exhibits reversible photoconversion between far-red-absorbing dark-adapted and orange-absorbing photoproduct states. Herein, we applied various biochemical and spectral studies to identify the details of the conformational change during this photoconversion process. No oligomeric state change was observed, whereas the surface charge would change with a modification of the α-helix structures during the photoconversion process. Combinatorial analysis using partial protease digestion and mass spectrometry identified the region where the conformational change occurred. These results provide clues for the future development of optogenetic tools. [Display omitted] • Biliverdin-binding AnPixJg2 variant, AnPixJg2_BV4 H318 was biochemically analyzed. • AnPixJg2_BV4 H318 exhibits surface charge change upon photoconversion. • Region of the conformational change was revealed upon photoconversion. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHYTOCHROMES
*SURFACE charges
*ADENYLATE cyclase
*COMBINATORICS
*MASS spectrometry
Subjects
Details
- Language :
- English
- ISSN :
- 00039861
- Volume :
- 745
- Database :
- Academic Search Index
- Journal :
- Archives of Biochemistry & Biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 170413183
- Full Text :
- https://doi.org/10.1016/j.abb.2023.109715