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N-terminal acetylation of the βC1 protein encoded by the betasatellite of tomato yellow leaf curl China virus is critical for its viral pathogenicity.
- Source :
-
Virology . Sep2023, Vol. 586, p1-11. 11p. - Publication Year :
- 2023
-
Abstract
- N-terminal acetylation (N -acetylation) is one of the most common protein modifications and plays crucial roles in viability and stress responses in animals and plants. However, very little is known about N -acetylation of viral proteins. Here, we identified the Thr residue at position 2 (Thr-2) in the βC1 protein encoded by the betasatellite of tomato yellow leaf curl China virus (TYLCCNB-βC1) as a novel N -acetylation site. Furthermore, the effects of TYLCCNB-βC1 N -acetylation on its function as a pathogenicity factor were determined via N -acetylation mutants in Nicotiana benthamiana plants. We found that N -acetylation of TYLCCNB-βC1 is critical for its self-interaction in the nucleus and viral pathogenesis, and that removal of N -acetylation of TYLCCNB-βC1 attenuated tomato yellow leaf curl China virus-induced symptoms and led to accelerated degradation of TYLCCNB-βC1 through the ubiquitin-proteasome system. Our data reveal a protective effect of N -acetylation of TYLCCNB-βC1 on its pathogenesis and demonstrate an antagonistic crosstalk between N -acetylation and ubiquitination in this geminiviral protein. • N-terminal acetylation (N -acetylation) is one of the most common protein modifications and plays crucial roles in viability and stress responses in animals and plants. • The Thr residue at position 2 (Thr-2) in the βC1 protein encoded by the betasatellite of tomato yellow leaf curl China virus (TYLCCNB-βC1) as a novel N -acetylation site. • N -acetylation of TYLCCNB-βC1 is critical for viral pathogenesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TOMATO yellow leaf curl virus
*NICOTIANA benthamiana
*ACETYLATION
*VIRAL proteins
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 586
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 170043312
- Full Text :
- https://doi.org/10.1016/j.virol.2023.07.003