高效反硝化聚磷菌的筛选及其脱氮除磷条件和性能研究 .

The objective is to explore the denitrification and phosphorus removal conditions and performance of a efficient denitrifying phosphorus-accumulating bacterium, and its treatment effect on simulated wastewater. Denitrifying phosphorus-accumulating strain D4 was isolated by pure culture technology. Preliminary identification of the strain was completed based on 16S rRNA gene sequence analysis and physiological and biochemical characteristics, and the denitrification and dephosphorization conditions were optimized by single factor and response surface method, so as to achieve efficient biological denitrification and dephosphorization of mimic food wastewater. As results， denitrifying phosphorus-accumulating bacterium D4 was Gordonia sp.. The optimal conditions for nitrogen and phosphorus removal were sodium acetate (3.32 g/L) as carbon source, 5% inoculum, initial phosphorus content of 18.61 mg/L, 31.3℃, and pH 7.9. Under these conditions, the removed amounts of total phosphorus, nitrate nitrogen and ammonia nitrogen by strain D4 were 14.18, 39.67 and 69.71 mg/L, respectively; and the corresponding removal rates were 84.50%, 97.67% and 96.22%. Results of treatment to different simulated wastewater showed that strain D4 had the best nitrogen and phosphorus removal effects in soybean product wastewater, with the removed amount of nitrogen and phosphorus being 101.58 mg/L and 10.40 mg/L, respectively. In conclusion, strain D4 has promising denitrification and phosphorus-accumulating ability and is a good strain resource for biological denitrification and phosphorus removal of food processing wastewater with high nitrogen and phosphorus such as soybean product wastewater. [ABSTRACT FROM AUTHOR]