Dual-channel MIRECL portable devices with impedance effect coupled smartphone and machine learning system for tyramine identification and quantification.

[Display omitted] • Smartphone-based multicolor MIRECL sensing system was proposed. • Visual and portable optical device integrated with machine learning was designed. • App based on yolov5x algorithm with five different linearting algorithms. • A smartphone-based handheld device for on-site and rapid detection TYM. We designed a novel, portable, and visual dual-potential molecularly imprinted ratiometric electrochemiluminescence (MIRECL) sensor for tyramine (TYM) detection based on smartphone and deep learning-assisted optical devices. Molecularly imprinted polymer-Ce 2 Sn 2 O 7 (MIP-Ce 2 Sn 2 O 7) layers were fabricated by in-situ electropolymerization method as the capture and signal amplification probe. Oxygen vacancies in Ce 2 Sn 2 O 7 not only enhance the electrochemical redox capability but also accelerate the energy transfer, thereby enhancing the luminescence of cathode ECL. Under optimal conditions, the ECL signals of MIP-Ce 2 Sn 2 O 7 at the cathode and the anode response of Ru(bpy) 3 2+ was reduced, thus a wide linear range from 0.01 μM to 1000 μM with the detection limit as low as 0.005 μM. Interestingly, combined with an artificial intelligence image recognition algorithm and the principle of optical signal reading by smartphone, the developed MIRECL sensor has been applied to the portable and visual determination of TYM in aquatic samples, and its practicability has been satisfactorily verified. [ABSTRACT FROM AUTHOR]