人参皂苷 Rd对新生鼠脑室周围白质软化模型学习记忆的影响.

Objective To investigate the effects of exogenous Ginsenoside Rd on the learing and memory in the brain of the neonatal periventricular leukomalacia (PVL) rat, in order to provide experimental evidence for clinical treatment of preterm infant with PVL. Methods In June 2021, A total of 120 2-day-old neonatal SD rats were selected as the experimental animals. All the experimental SD rats were caged and divided into the sham operation group, the PVL group (control group) and the PVL Ginsenoside Rd intervention group (Ginsenoside Rd intervention group) according to the principle of randomization, with 40 rats in each group. The PVL animal model was prepared by ligation of the left common carotid artery combined with hypoxia (8%O2+92%N2) for half an hour. The pathological changes of periventricular white matter area of the rats in the three groups were observed on 4, 7 and 14 days after the operation. Morris water maze experiment was conducted on 30 days after birth to explore the learning and memory ability of the rats in the three groups. Results No abnormal pathological changes were found in the white matter area of the sham operation group 4, 7 and 14 days after operation. In the control group, the tissue structure of the white matter area of the rats was obviously loose 4 days after operation, with cell swelling, necrosis and nuclear shrinkage. Both the control group and the Ginsenoside Rd intervention group had liquefaction necrosis and cyst formation in the periventricular white matter area 7 days after operation, and the left ventricle was slightly enlarged. 14 days after operation, the left ventricle of the control group was further enlarged, and the white matter around the ventricle was obviously softened. The changes of periventricular white matter in the Ginsenoside Rd intervention group were similar to those in the control group 4 to 14 days after operation, but the pathological changes of white matter were significantly lighter than those in the control group at each time point. At the same training time 30 days after birth, the escape latency of the three groups was statistically significant (P＜0. 05). There were significant differences in escape latency 3 days before training, 3 days after training and 5 days after training (P＜0. 05). There was no significant difference in escape latency between the three groups at the 4th and 5th day (P＞0. 05). There was statistical significance in the number of times of the three groups of rats passing through the circle within 120 s, that was the piercing index (P＜0. 05). Conclusion Ginsenoside Rd can reduce the damage of periventricular white matter and improve the learning and memory ability of PVL rats. [ABSTRACT FROM AUTHOR]