Assessment of level of FSH and Inhibin B in the evaluation of primary male infertility with reference to spermiogram.

Globally, 48 million couples and 186 million individuals face the problem of infertility. Out of all infertility cases, approximately 40-50% are due to the "male factor" and 2% of all men will exhibit suboptimal sperm parameters. It may be one or a combination of low sperm concentration, poor sperm motility, or abnormal morphology. In recent years, the development of computerized systems provides an objective and rapid method for semen analysis, suitable for the study of more sophisticated parameters of sperm motility. Electron microscopy should be performed for the evaluation of ultrastructural abnormalities of spermatozoa in men with infertility of uncertain origin. Method Semen was examined macroscopically as well as under the microscope as per WHO guidelines for the count, vitality, and morphology by using routine microscopy with PAP stain and Eosin nigrosin dye. The FSH and Inhibin B test was performed using sandwich Elisa by commercially available kit (Qualisa™ FSH) kit and AL-107-i. CE kit respectively. Result The majority of patients were seen in the age group of 26-30 years. Total motility of sperms, progressive motile sperms, and semen vitality between 11-20 % was seen in a maximum of 33, 30, and 24 patients respectively. 26 cases had a normal form of sperm between 0-5%. The FSH level and Inhibin B level were lowest and highest at 16-20% of sperm count. Conclusion Infertility is one of the biggest causes of stress among married couples. The parameters of male infertility can be easily diagnosed by a simple semen examination. The FSH and Inhibin B-level investigations provide a better mode of treatment for male infertility. [ABSTRACT FROM AUTHOR]