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Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.

Authors :
Luo, Tongyun
Li, Lujing
Wang, Shirui
Cheng, Nan
Source :
International Journal of Molecular Sciences. Aug2023, Vol. 24 Issue 15, p12247. 23p.
Publication Year :
2023

Abstract

Genetically modified (GM) maize is one of the earliest GM crops to have achieved large-scale commercial cultivation globally, and it is of great significance to excel in the development and implementation of safety policy regarding GM, and in its technical oversight. This article describes the general situation regarding genetically modified maize, including its varieties, applications, relevant laws and regulations, and so on. From a technical point of view, we summarize and critically analyze the existing methods for detecting nucleic acid levels in genetically modified maize. The nucleic acid extraction technology used for maize is explained, and the introduction of traditional detection techniques, which cover variable-temperature and isothermal amplification detection technology and gene chip technology, applications in maize are described. Moreover, new technologies are proposed, with special attention paid to nucleic acid detection methods using sensors. Finally, we review the current limitations and challenges of GM maize nucleic acid testing and share our vision for the future direction of this field. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
*NUCLEIC acids

Details

Language :
English
ISSN :
16616596
Volume :
24
Issue :
15
Database :
Academic Search Index
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
169926727
Full Text :
https://doi.org/10.3390/ijms241512247