Back to Search
Start Over
Cyanine‐ and Rhodamine‐Derived Alkynes for the Selective Targeting of Cancerous Mitochondria through Radical Thiol‐Yne Coupling in Live Cells.
Cyanine‐ and Rhodamine‐Derived Alkynes for the Selective Targeting of Cancerous Mitochondria through Radical Thiol‐Yne Coupling in Live Cells.
- Source :
-
Chemistry - A European Journal . 8/10/2023, Vol. 29 Issue 45, p1-12. 12p. - Publication Year :
- 2023
-
Abstract
- Despite their long history and their synthetic potential underlined by various recent advances, radical thiol‐yne coupling reactions have so far only rarely been exploited for the functionalization of biomolecules, and no examples yet exist for their application in live cells ‐ although natural thiols show widespread occurrence therein. By taking advantage of the particular cellular conditions of mitochondria in cancer cells, we have demonstrated that radical thiol‐yne coupling represents a powerful reaction principle for the selective targeting of these organelles. Within our studies, fluorescently labeled reactive alkyne probes were investigated, for which the fluorescent moiety was chosen to enable both mitochondria accumulation as well as highly sensitive detection. After preliminary studies under cell‐free conditions, the most promising alkyne‐dye conjugates were evaluated in various cellular experiments comprising analysis by flow cytometry and microscopy. All in all, these results pave the way for improved future therapeutic strategies relying on live‐cell compatibility and selectivity among cellular compartments. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RADICALS (Chemistry)
*ALKYNES
*FLOW cytometry
*CANCER cells
*ORGANELLES
Subjects
Details
- Language :
- English
- ISSN :
- 09476539
- Volume :
- 29
- Issue :
- 45
- Database :
- Academic Search Index
- Journal :
- Chemistry - A European Journal
- Publication Type :
- Academic Journal
- Accession number :
- 169875215
- Full Text :
- https://doi.org/10.1002/chem.202301340