Carbapenem Resistance in Clinical Isolates of Escherichia Coli by Phenotypic and Genotypic Method among Patients Visiting a Tertiary Care Hospital, in Central India.

Introduction: The emergence and increase of Carbapenem resistance in Escherichia coli is now posing a serious threat to human health around the world. Our study aimed to investigate the phenotypic and genotypic detection of Carbapenem resistance among E. coli isolates. Material Method: investigated irrespective of age, sex, departments and Carbapenemase resistance among various clinical samples such as urine, pus, blood, CSF and respiratory secretions received in microbiology laboratory. Results: It is observed that the total 215 isolates of E. coli were included in this study. About 153 (71.16%) isolates were from male patients while 62 (28.83%) were from female patients. The majority (53.95%) of isolates were from urine samples. Out of 215 E. coli isolates, total 63 (29.30%) were carbapenem resistant. Out of 63 carbapenem resistant isolates, maximum carbapenem resistance was found 59 isolates (12.20%) were having Oxa48 gene, 27 NDM (12.55%), 3 E. coli isolates were having gene KPC and 2 E.coli strains were found with both KPC & NDM gene. Conclusion: Carbapenem resistant E.coli are increasing rapidly and becoming major problem in the area of infectious diseases. Early detection of changing resistance patterns is very important in preventing the dissemination of resistant bacteria and modifying the treatment strategies. [ABSTRACT FROM AUTHOR]