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Calcium channels are present in the apical plasma membranes of the hepatopancreatic B-cells of Marsupenaeus japonicus.
- Source :
-
Journal of Comparative Physiology B: Biochemical, Systemic & Environmental Physiology . Nov2003, Vol. 173 Issue 8, p661-667. 7p. - Publication Year :
- 2003
-
Abstract
- This study demonstrates the existence of calcium channels in the apical membranes of the hepatopancreatic blister (B) cells of Marsupenaeus japonicus. Using brush-border membrane vesicles we demonstrated that the channel-mediated calcium passive flux was saturable and was stimulated by a transmembrane electrical potential difference and inhibited by barium. We raised a monoclonal antibody (Mab 24A4) against the calcium channel, which allowed us to inhibit the channel-mediated calcium uptake. By immunocytochemistry, using Mab 24A4, we demonstrated that these channels are located at the apical membrane of hepatopancreatic B cells. Finally, by measuring the calcium uptake in R- and B-enriched cell suspensions, we showed that only the plasma membrane of the B cells expresses a channel-mediated calcium uptake inhibited by barium, verapamil and the monoclonal antibody 24A4. The plasma membrane of R cells did not show calcium channels. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01741578
- Volume :
- 173
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Journal of Comparative Physiology B: Biochemical, Systemic & Environmental Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 16984971
- Full Text :
- https://doi.org/10.1007/s00360-003-0375-3