Anti-diabetic effect of interruptins A and B from Cyclosorus terminans on mouse hepatocytes.

Interruptin B from the fern Cyclosorus terminans has been indicated as a ligand of peroxisome proliferator-activated receptor (PPAR)-α and -γ that is known to regulate glucose homeostasis. Interruptin B was previously been implicated to promote glucose consumption into adipocytes. This study aimed to examine the anti-diabetic impact of interruptins A and B on glucose uptake and glycogen synthesis in mouse hepatocytes. Cell viability of FL83B hepatocytes affected by interruptins A and B was determined. The glucose consumption after 12-24 h treatment with compounds was evaluated by glucose oxidase method. The glycogen content was assessed after 24 h incubation using anthrone method. The mechanism regarding glucose consumption was investigated by coincubation of interruptins A or B with PPAR-γ antagonist bisphenol A diglycidyl ether (BADGE) and by protein expression on PPAR-γ, -α, glucose transporter 2 (GLUT2), and phosphorylated-insulin receptor substrate-1 (P-ISR-1) through Western blotting technique. Our study demonstrated that interruptins A and B were non-toxic toward FL83B hepatocytes. They remarkably stimulated glucose consumption and glycogen storage in a time- and dose-dependent manner. However, their increased glucose uptake was diminished by BADGE treatment. Moreover, interruptins A and B stimulated protein expression of PPAR-γ, -α, GLUT2, and phosphorylated-IRS-1. These findings support the anti-diabetic potential of interruptins A and B by modulation of the PPARs pathway. [ABSTRACT FROM AUTHOR]