In-vitro and in-vivo antimicrobial activity of Citrullus lanatus prolamin hydrolysates.

• Prolamin hydrolysate of C. lanatus rich in amino acids serve as good source of nutrition. • The hydrolysate of the prolamin from C. lanatus exhibited remarkable antibacterial action against Gram-negative infections. • The prolamin hyrolysate of C. lanatus shows potential role in eliminating P. aeruginosa in the animal model. In recent years, the exploration of novel antibiotic agents from natural sources has gained significant attention in today's research due to a rapid increase in resistance to the existent synthetic drugs. The antimicrobial activity of prolamin hydrolysate of Citrullus lanatus (CLPH) was carried out using two different in-vitro antimicrobial methods (viable cell count method and broth-based turbidometric assay) against both Gram-positive and Gram-negative bacteria. The in-vivo antibacterial effect of CLPH against Pseudomonas aeruginosa was evaluated in detail using the animal model. The total protein content and amino acid compositions of CLPH was also determined. CLPH exhibited high efficacy towards Gram-negative bacteria and inhibited the growth of P. aeruginosa (37±3.1 µg/ml). The CLPH shows a potential role in eliminating P. aeruginosa in the animal model. Among all identified amino acids, arginine content in CLPH was found to be 189.4 mg/100 g. P. aeruginosa is a Gram-negative bacterium, which develops multi-drug resistance and enhances antibiotic resistance rate. Therefore, this outcome may have significant importance in the development of novel antibiotics from the food source for the treatment of bacterial infections. [ABSTRACT FROM AUTHOR]