Investigation of Antioxidant Enzymes and Some Biochemical Parameters in Ewes with Gangrenous Mastitis.

This paper investigates the effects of gangrenous mastitis seen with cell necrosis and tissue damage on the activity of erythrocyte glutathione peroxidase (GSH-Px), level of plasma lipid peroxidation (MDA), and some biochemical parameters. To do that, 20 clinically gangrenous mastitis diagnosed and 20 healthy ewes, all from the same flock in a local ewes unit were used as material. Blood samples were kept in test tube containing the disodium salt of ethylenediamine tetraacetic acid (EDTA) as anticoagulant for plasma MDA and erythrocyte GSH-Px analysis and in vacutainer tube for biochemical analyses from the jugular vein. The following measurements were performed in blood serum: glucose, cholesterol, triglyceride, total protein, albumin, sodium (Na), potassium (K), chloride (Cl), calcium (Ca), total bilirubin, alanine transaminase (ALT), aspartate transaminase (AST), urea and creatinine in an autoanalyzer. Erythrocyte GSH-Px activities and plasma MDA levels were found to be significantly higher in the ewes with gangrenous mastitis than healthy ewes. While there was an increase in creatinine and total bilirubin, Na, K, Ca, glucose and albumin were significantly decreased in the gangrenous mastitic group than the control group. In conclusion, in ewes, the severe damage in the inflamed mammary gland tissue during gangrenous mastitis might be said to cause high oxidant stress, which leads to increases in the levels of erythrocyte GSH-Px and plasma MDA. [ABSTRACT FROM AUTHOR]