Aspects of studying the mycobiota biofilm of the oil pipeline soil ecosystem during scanning electron microscopy.

The relevance of studying the microbial factor in assessing the corrosion activity of the soil near the oil pipeline is confirmed by a number of studies by the authors. The existence of microbial populations in the form of biofilms colonizing the soil substrates of the pipeline represents a certain strategy for their survival in an unfavorable medium. The purpose of the study was to analyze the features of a biofilm formation by micromycetes of the pipeline soil ecosystem during scanning electron microscopy. At the first stage, to study the biocorrosive activity of soil samples, we determined the quantitative content of thionic and sulfate-reducing bacteria, the total concentration of sulfur, iron, and the value of the electrical resistivity of the soil of the underground pipeline. The criterion of biocorrosive activity of the soil was established, which determines the total effect of various corrosion factors. At the second stage, we carried out a mycological study and obtained a micromycetes culture. At the third stage of the research, in its experimental part, the biofilm on the surface of steel samples was studied using electron microscopy as well as qualitative and quantitative elemental chemical analysis. The results of the soil biocorrosive activity studies proved the presence of aggressive, moderately aggressive, mildly aggressive, and potentially aggressive soil samples; there were no very aggressive samples. During the bacteriological analysis of the samples, thionic and sulfate-reducing bacteria and cultures of micromycetes of the genera Penicillium and Aspergillus were identified. During electron microscopy, the areas of the developed biofilm of mycobiota and formed microcracks on the surface of the samples were found, qualitative and quantitative elemental analysis was carried out. [ABSTRACT FROM AUTHOR]