اثر نانوسامانه نیوزومی حاوي جنتامیسین بر باکتري استافیلوکوکوس اورئوس: یک مطالعه آزمایشگاهی

Background and Objectives: Antibiotic resistance is known to be the main challenge against treatment of infectious diseases related to Staphylococcus aureus (S.aureus). Niosomal nanosystems are the new drug delivery systems which enhance antimicrobial potential activity of antibiotics. The aim of this study was to determine the effect of a niosomal drug delivery system containing gentamicin against S.aureus. Materials and Methods: In this laboratory study, different niosomes including Span 60, Tween 60, and cholesterol in combination with gentamicin with different molar ratio were synthesized using the thin film hydration method. Synthesized niosomal nanosystems were identified by light microscope and dynamic laser light scattering (DLS) technique. Antibacterial activity of synthesized niosomes against S.aureus (PTCC: 1112) was evaluated by well diffusion and minimum inhibitory concentration (MIC) methods. Data was analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc test. ResultsNiosomal nanosystems were identified in spherical vesicles and in 3 formulations with average particle sizes of 9.09±0.76, 27.59±15.20 and 9.10±0.30 μm, respectively. The results revealed that the formulation with 60:40 molar ratio had higher gentamycin encapsulation efficiency (90.82%) and also, 60:40 niosomal formulation with half the concentration of commercially available gentamicin solution had the same inhibition of growth on Staphylococcus aureus bacteria (p=0.658) . ConclusionControlled and continuous release of gentamicin from the niosomal nanosystem, along with enhanced drug penetration could reduce the growth of S.aureus. Accordingly, niosomal nanosystem has a good potential to be an effective drug delivery system. [ABSTRACT FROM AUTHOR]