Isolation Of Pseudomonas Fluorescens From Erbil Soils And Screening For The Presence Of Siderophores Biosynthesis Genes.

The current study was aimed to isolate and identify Fluorescent Pseudomonads from Erbil soil and then screening for Siderophores biosynthesis genes. Soil samples were collected from different location in Erbil governorate. A total of 18 isolates of Pseudomonas fluorescens were isolated on enriched and selective King B medium and they identified using API20E, Vitek2 morphological features, cultural, biochemical characteristics, and 16S rRNA gene sequencing technique. Plant growth promoting traits of the isolates were also studied such as IAA, siderophore and HCN production. All isolates exhibited high potential of IAA, HCN and siderophore production. We selected different isolate for screen the presence of Iron solubilizing gene using specific primers with PCR technique. All isolates resolute for their iron solubilizing efficiency and all of them showed iron solubilizing activity. Isolate (Cpf3) were the most efficient isolate, while (Dpf16) were the least efficient isolates in Fe-solubilization. psiB and psiG genes were selected to screen for the presence of iron solubilizing using specific primers with PCR technique. The results showed positive reactions and all isolates contained iron solubilizing genes. [ABSTRACT FROM AUTHOR]