Developing a UV–visible reporter‐assisted CRISPR/Cas9 gene editing system to alter flowering time in Chrysanthemum indicum.

Keywords: Chrysanthemum; CRISPR/Cas9; eYGFPuv; flowering time; TFL1 EN Chrysanthemum CRISPR/Cas9 eYGFPuv flowering time TFL1 1519 1521 3 07/26/23 20230801 NES 230801 Chrysanthemum ( I Chrysanthemum morifolium i Ramat.) Developing a UV-visible reporter-assisted CRISPR/Cas9 gene editing system to alter flowering time in Chrysanthemum indicum When I CiPDS i is used as a visual marker to validate genome editing, chimerism is easily scorable, but scoring is time-consuming and laborious for most target genes without a visual mutant phenotype. In this regard, CRISPR/Cas9-mediated genome editing holds advantages in vector design and assembly, especially when targeting multiple genes, and has been widely used in many organisms. [Extracted from the article]