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BG01V: A variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation.
- Source :
-
Restorative Neurology & Neuroscience . 2004, Vol. 22 Issue 6, p421-428. 8p. - Publication Year :
- 2004
-
Abstract
- Purpose: To explore a karyotypically abnormal variant human embryonic stem cell (hESC) line, BG01V, as a potential model for studies of dopaminergic neuronal differentiation. Methods: The properties of BG01V cells were compared to those of normal BG01 cells using immunocytochemistry, RT-PCR, focused microarrays and in vitro differentiation, including dopaminergic differentiation, by culturing with the stromal cell line PA6. Results: Despite the karyotypic abnormality (49, +12, +17 and XXY), undifferentiated BG01V cells expressed pluripotent ESC markers similar to BG01 cells, and retained the ability to differentiate into cell types characteristic of all three germ layers. When co-cultured with the stromal cell line PA6, BG01V cells differentiated into dopaminergic cells which exhibited properties similar to those of mature dopaminergic neurons. Conclusions: BG01V cells were easier to maintain in culture than karyotypically normal BG01 cells and can be used as an alternative pluripotent hESC type for in vitro developmental studies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09226028
- Volume :
- 22
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Restorative Neurology & Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 16573820