The inhibition of the viability of myeloma cells and the production of cytokine by two strains ofLactobacillus sakeifrom meat.

Ten strains of lactic acid bacteria were isolated from a dry fermented sausage and tested for stimulation or inhibition of the viability of Vero and myeloma cells. They did not significantly affect the viability of Vero cells but two isolates (CBL/H and CBL/K) showed a strong and moderate inhibition of the myeloma cell viability (at 108 CFU mL−1, 17.6 and 33.2%, respectively, survival of myeloma cells). The isolates were identified asLactobacillus sakeiby DNA sequencing of the 16S rRNA products of a polymerase chain reaction. No protective effect was found, at the concentrations used, against cytotoxicity of N-nitrosamines. To test the effect ofL. sakeiCBL/H and CBL/K on cytokine production [tumour necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-8 (IL-8)], the human macrophage cell line (THP-1) was cultured in the presence and absence of lipopolysaccharide (LPS).Lactobacillus sakeiCBL/H and CBL/K induced IL-1β and IL-8 release when cells were stimulated with and without LPS. However there was TNF-α release only in the presence of the LPS. [ABSTRACT FROM AUTHOR]