基于低共熔溶剂高效萃取液质联用快速定量检测 养殖水中氯霉素类抗生素残留方法的研究.

To establish a rapid and quantitative method for the determination of chloramphenicol antibiotics residues in aquaculture water by liquid chromatography mass spectrometry (LC- MS) with micro eutectic solvent extraction and enrichment, the culture water was filtered by filter paper, 10 mL of water sample was added with proper amount of internal standard substance, 1 mL of water sample was mixed evenly in a micro-centrifuge tube, adding proper amount of eutectic solvent, vortex mixing and standing to extract the top layer clear liquid, acetonitrile was fixed to 1 mL, and filtered by 0.22 μm. The sample was separated by high- performance liquid chromatography, and the internal standard was quantified by electrospray ionization tandem mass spectrometry. The results showed the linear range of chloramphenicol was 0.1-5.0 μg·kg-1, and the correlation coefficient was 0.999 4. The average detection limit of chloramphenicol was up to 0.005 3 μg · kg-1 . The recovery of chloramphenicol was 80.3%-103.6%, the relative standard deviation was between 3.5% and 8.3%. The linear range of the florfenicol was 1.0-20.0 μg·kg-1, and the correlation coefficient was 0.999 8. The average detection limit of the florfenicol was up to 0.022 8 μg·kg-1 . The recoveries of the method ranged from 63.4% to 85.2%, the relative standard deviation was between 2.2% and 6.2%. The results showed that method had the advantages of less consumption of extraction and detection reagent, short time, high sensitivity and good accuracy. This method was proved to be suitable for the detection of chloramphenicol in aquaculture water, and provided a clear research direction for the next step of using low eutectic solvent extraction in aquaculture environment and detection of veterinary drug residues in aquatic products. [ABSTRACT FROM AUTHOR]