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A Blue Light and Two‐Photon Activatable Rhodamine Fluorophore.
- Source :
-
Chemistry - A European Journal . 7/6/2023, Vol. 29 Issue 38, p1-6. 6p. - Publication Year :
- 2023
-
Abstract
- Photoactivatable fluorophores (PAFs) are powerful tools for biological imaging applications because they provide spatiotemporal control of fluorescence distribution. Many of the existing PAFs can only be activated by UV irradiation. In our study, we present a blue light (1P) and NIR light (2P) activatable rhodamine fluorophore. Next to the description of the synthesis and the investigation of the photoreaction, we demonstrate the use of our PAF in the context of laser scanning microscopy. By immobilization of our PAF in a hydrogel, we were able to write and read spatially resolved illumination patterns with excellent contrast after both one‐photon and two‐photon excitation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BLUE light
*FLUOROPHORES
*LASER microscopy
*FLUORESCENCE
*PHOTOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 09476539
- Volume :
- 29
- Issue :
- 38
- Database :
- Academic Search Index
- Journal :
- Chemistry - A European Journal
- Publication Type :
- Academic Journal
- Accession number :
- 164764004
- Full Text :
- https://doi.org/10.1002/chem.202300997