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μPESI-MS/MS System for Screening and Quantitating Drugs in Plasma Samples.
- Source :
-
Journal of the American Society for Mass Spectrometry . 7/5/2023, Vol. 34 Issue 7, p1295-1304. 10p. - Publication Year :
- 2023
-
Abstract
- Recently, we developed a novel microprobe electrospray ionization (μPESI) source and its coupled MS (μPESI-MS/MS) system. Here, we aimed to widely validate the μPESI-MS/MS method for quantitative analysis of drugs in plasma samples. Furthermore, the relationship between the quantitative performance of the μPESI-MS/MS method and the physicochemical properties of target drugs was analyzed. The μPESI-MS/MS methods for quantitative analysis of 5 representative drugs with a relatively wide range of molecular weight, pKa, and log P values were developed and validated. The results showed that the linearity, accuracy, and precision of these methods met the requirements of the European Medicines Agency (EMA) guidance. Then a total of 75 drugs from plasma samples were primarily detected using the μPESI-MS/MS methods, among which 48 drugs could be quantitatively measured. Logistics regression suggested that drugs with significantly greater log P and physiological charge had better quantitative performance using the μPESI-MS/MS method. Collectively, these results clearly demonstrate the practical application of the μPESI-MS/MS system as a rapid approach to the quantitative analysis of drugs in plasma samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10440305
- Volume :
- 34
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of the American Society for Mass Spectrometry
- Publication Type :
- Academic Journal
- Accession number :
- 164761225
- Full Text :
- https://doi.org/10.1021/jasms.3c00025