بر سلولهای بنیادی اسپرم ساز، سلولهای BMP و GATA ، اثر بیان آلکالین فسفاتاز C57BL جنینی و شبه سلولهای بنیادی جنینی در موشهای نژاد.

Background and Objective: Alkaline phosphatase, BMP, and GATA proteins are important factors in the process of spermatogenesis. This study aims to investigate the effect of alkaline phosphatase, GATA, and BMP expression on spermatogenic stem cells, embryonic cells, and embryonic stem-like cells (ES-like) in C57BL mice. Methods: In this experimental study, spermatogonial stem cells were isolated from three heads of 4-week-old C57BL mice, and embryonic stem cells and ES-like cells were prepared. Alkaline phosphatase staining test was performed on spermatogenic stem cells, embryonic cells, and ES-like cells. The expression of BMP and GATA genes was analyzed using Fluidigm PCR. Protein-protein interaction networks were isolated and drawn using databases. Results: Positive alkaline phosphatase expression in stem cells and negative expression in testicular Sertoli cells indicated the presence of this enzyme in pluripotent cells. The gene expression of BMP and GATA in spermatogonial stem cells (6.3 and 2.7, respectively), embryonic cells (3.2 and 4.4, respectively), and ES-like cells (8.5 and 2.5, respectively) was positive, but not statistically significant. Bioinformatics studies showed the regulatory role of these genes and their direct effect on alkaline phosphatase. Conclusion: BMP and GATA genes, along with alkaline phosphatase enzymes, play a crucial role in controlling embryonic and spermatogonial stem cells, maintaining their pluripotency, and guiding them towards differentiated cells. [ABSTRACT FROM AUTHOR]