Creation of Yersinia immunoassay test system based on antibodies to dimethyl sulfoxide antigen Yersinia pseudotuberculosis.

An ELISA test system was created based on antibodies obtained as a result of immunization of rabbits and guinea pigs with the dimethyl sulfoxide antigen of Yersinia pseudotuberculosis. It showed high sensitivity and generic specificity with all strains of enteropathogenic Yersinia and did not interact with bacteria from other genera, which allows it to be used for diagnostic purposes. In accumulation media with pig faeces contaminated with Yersinia, the experimental test system detected Y. pseudotuberculosis and Yersinia enterocolitica already on the 3rd day of "cold enrichment", provided that 50 or more Yersinia cells per 1 ml were inoculated into the accumulation medium together with feces. [ABSTRACT FROM AUTHOR]