Influence of graphene on different pathways of Castanopsis hystrix roots based on transcriptome analysis.

In order to investigate the molecular mechanism and transcriptomics of the response of graphene to the roots of Castanopsis hystrix Miq plant. Seedlings were cultivated with 4 groups of graphene solution concentrations of 0, 25, 33 and 50 mg/L, 0 refers to distilled water culture, the root development, gene expression, enrichment pathway and range of differentially expressed genes were analyzed. Compared with 0 group, 25 mg/L graphene solution had the best promoting effect on root development of Castanopsis hystrix seedlings(t=2.86 to 7.80, P<0.05). Transcriptomic data showed that the expression levels of 12 845 genes were changed after 25 mg/L graphene solution was applied to Castanopsis hystrix roots, among which 10 899 genes were up-regulated and 1 946 genes were inhibited. According to the Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis, the up-regulated genes were mainly enriched in 126 KEGG pathways, and down-regulated genes were enriched in 99 KEGG pathways. In addition, 110 transcription factor genes were differentially expressed and could be classified into 33 different families. Among them, 7 transcription factor family genes, including ERF, WRKY, MYB, NAC, bHLH, TCP and Trihelix were related to root development of Castanopsis hystrix. Analyses of differentially expressed genes related to plant hormone signal transduction pathways showed changes in the expression levels of 7 hormone-related genes, including auxin, cytokinin, gibberellin, ethylene, salicylic acid, brassinosteroid and strigolactone, were closely related to the root growth of Castanopsis hystrix. [ABSTRACT FROM AUTHOR]