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A portable, high-throughput real-time quantitative PCR device for point-of-care testing.
- Source :
-
Analytical Biochemistry . Aug2023, Vol. 674, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Nucleic acids detection has become essential in the identification of many infectious diseases and tumors. Conventional qPCR instruments are not suitable for point-of-care Moreover, current miniaturized nucleic acid detection equipment has limited throughput and multiplex detection capabilities, typically allowing the detection of a limited number of samples. Here, we present an affordable, portable, and high-throughput nucleic acid detection device for point-of-care detection. This portable device is approximately 220 × 165 × 140 mm in size and about 3 kg in weight. It can provide stable and accurate temperature control and analyze two fluorescent signals (FAM and VIC) and run 16 samples simultaneously. As a proof of concept, we used the two purified DNA samples from Bordetella pertussis and Canine parvovirus and the results showed good linearity and coefficient of variation. Moreover, this portable device can detect as low as 10 copies and has good specificity. Therefore, our device can provide advantages in real-time diagnosis of high-throughput nucleic acid detection in the field, especially for resource-limited conditions. [Display omitted] • An affordable, portable, and high-throughput nucleic acid detection device was developed. • The device can analyze two fluorescent signals (FAM and VIC) and run 16 samples simultaneously. • This portable device can provide advantages in real-time diagnosis of high-throughput nucleic acid detection in resource-limited conditions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 674
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 164377861
- Full Text :
- https://doi.org/10.1016/j.ab.2023.115200