Back to Search Start Over

Ectopic ATP synthase stimulates the secretion of extracellular vesicles in cancer cells.

Authors :
Kao, Yi-Chun
Chang, Yi-Wen
Lai, Charles P.
Chang, Nai-Wen
Huang, Chen-Hao
Chen, Chien-Sheng
Huang, Hsuan-Cheng
Juan, Hsueh-Fen
Source :
Communications Biology. 6/15/2023, Vol. 6 Issue 1, p1-17. 17p.
Publication Year :
2023

Abstract

Abstarct: Ectopic ATP synthase on the plasma membrane (eATP synthase) has been found in various cancer types and is a potential target for cancer therapy. However, whether it provides a functional role in tumor progression remains unclear. Here, quantitative proteomics reveals that cancer cells under starvation stress express higher eATP synthase and enhance the production of extracellular vesicles (EVs), which are vital regulators within the tumor microenvironment. Further results show that eATP synthase generates extracellular ATP to stimulate EV secretion by enhancing P2X7 receptor–triggered Ca2+ influx. Surprisingly, eATP synthase is also located on the surface of tumor-secreted EVs. The EVs-surface eATP synthase increases the uptake of tumor-secreted EVs in Jurkat T-cells via association with Fyn, a plasma membrane protein found in immune cells. The eATP synthase-coated EVs uptake subsequently represses the proliferation and cytokine secretion of Jurkat T-cells. This study clarifies the role of eATP synthase on EV secretion and its influence on immune cells. eATP synthase is expressed on the surface of cancer-derived extracellular vesicles (EVs), contributes to EV uptake and reduces Jurkat T cell proliferation and cytokine release. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23993642
Volume :
6
Issue :
1
Database :
Academic Search Index
Journal :
Communications Biology
Publication Type :
Academic Journal
Accession number :
164356490
Full Text :
https://doi.org/10.1038/s42003-023-05008-5