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Antioxidation, immunity and hepatopancreatic histology of red swamp crayfish (Procambarus clarkii) subjected to low-temperature aerial exposure stress and re-immersion.
- Source :
-
Comparative Biochemistry & Physiology Part A: Molecular & Integrative Physiology . Aug2023, Vol. 282, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Desiccation is a stressful situation that decapods often experience during live transportation. This study investigated the effects of low-temperature aerial exposures (LTAEs) (dry exposure (DL) and moist exposure (ML) at 6 °C) and re-immersion on the antioxidative and immune responses and hepatopancreatic histopathology in P. clarkii. Compared to the control group (normally feeding at 24.0 °C water temperature), the crayfish under LTAEs showed overall severe hepatopancreatic oxidative damage, with significantly increased malondialdehyde (MDA) contents and significantly reduced total antioxidant capacity (T-AOC), and oxidant damage was not fully recovered even after 12 h of re-immersion; the expression of hsp70 was significantly increased within 24–48 h of stress and re-immersion. The activity of hemolymphatic acid phosphatase (ACP) was significantly increased during 24–48 h of the stress and at 12 h of re-immersion; the activity of aspartic aminotransferase (AST) and alanine aminotransferase (ALT) was significantly increased throughout the experiment; and the gene expression of proPO or TLR was significantly increased during 12–48 h of the stress. Severe histopathological changes (lumen dilatation, vacuolation of epithelial cells and reduced cell numbers) were observed in hepatopancreas at 48 h of stress and 12 h of re-immersion. These results indicated that 48 h of low-temperature aerial exposure stress stimulated the non-specific immunity but adversely affected the antioxidation and hepatopancreatic histomorphology of P. clarkii , whereas 12 h of re-immersion was not sufficient to restore crayfish from stress to a normal state. [Display omitted] • Low-temperature aerial exposure caused serious oxidant damage and stimulated the non-specific immune defenses. • Low-temperature aerial exposure severely impaired the hepatopancreatic histomorphology and physio-biochemical functions. • 12 h of re-immersion was not enough to fully recuperate from the 48 h of low-temperature aerial exposure stress. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRAYFISH
*PROCAMBARUS clarkii
*OXIDANT status
*ACID phosphatase
*HISTOLOGY
*SWAMPS
Subjects
Details
- Language :
- English
- ISSN :
- 10956433
- Volume :
- 282
- Database :
- Academic Search Index
- Journal :
- Comparative Biochemistry & Physiology Part A: Molecular & Integrative Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 164256805
- Full Text :
- https://doi.org/10.1016/j.cbpa.2023.111441