Back to Search
Start Over
Specific Features of the Determination of Xanthophyll Esters under Reversed-Phase HPLC Conditions.
- Source :
-
Journal of Analytical Chemistry . Jun2023, Vol. 78 Issue 6, p759-765. 7p. - Publication Year :
- 2023
-
Abstract
- Specific features of chromatographic determination of xanthophyll esters are studied using an example of marigold flower lutein diesters under reversed-phase HPLC conditions. The developed two-column method made it possible to establish that, in samples with a low solubility of carotenoids in the used solvent and on a chromatography column in using a mobile phase with a low solubility of carotenoids, the precipitation of diesters is possible, which detrimentally affects the accuracy of the chromatographic determination. A critical factor in this case isì temperature: the storage of samples (solutions) in a refrigerator is not always advisable, because freezing of the main components is possible. It was shown that the use of a mobile phase containing from 0 to 10 vol % acetonitrile in acetone at a temperature not lower than 20°C is acceptable for the separation of all-trans-lutein diesters from cis-derivatives of lutein and zeaxanthin derivatives on "monomeric" C18-phases. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10619348
- Volume :
- 78
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 164131687
- Full Text :
- https://doi.org/10.1134/S1061934823060023