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Thorough research and modification of one-carbon units cycle for improving L-methionine production in Escherichia coli.
- Source :
-
3 Biotech . Jun2023, Vol. 13 Issue 6, p1-10. 10p. - Publication Year :
- 2023
-
Abstract
- Methionine is the only one of the essential amino acids that contain sulfur, widely used as a feed additive in agriculture. In this study, the availability of 5-methyl-tetrahydrofolate was confirmed as the main limitation in the complex multibranched biosynthetic pathway of L-methionine. The cycle of one-carbon units was thoroughly investigated and modified to supply 5-methyl-tetrahydrofolate for L-methionine production, such as enhancing the supply of precursor, expediting the conversion rate of the cycle, introducing exogenous serine hydroxymethyltransferase and increasing pool size of one-carbon units carrier. The final strain MYA/pAmFA-4 was able to produce 20.89 g/L L-methionine by fed-batch fermentation, which was the highest titer reported in the literatures. This study is instructive for other metabolites biosynthesized needing one-carbon units or having a complex multibranched biosynthetic pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ESCHERICHIA coli
*METHIONINE
*ESSENTIAL amino acids
*FEED additives
Subjects
Details
- Language :
- English
- ISSN :
- 2190572X
- Volume :
- 13
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- 3 Biotech
- Publication Type :
- Academic Journal
- Accession number :
- 164080031
- Full Text :
- https://doi.org/10.1007/s13205-023-03625-9