Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl‐CoA synthetase inhibitor.

The compound ethyl‐adenosyl monophosphate ester (ethyl‐AMP) has been shown to effectively inhibit acetyl‐CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this study, the addition of ethyl‐AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co‐crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl‐AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins. [ABSTRACT FROM AUTHOR]