天麻素对儿童多发性抽动症模型大鼠 5-羟色胺系统的影响及相关机制.

Objective To evaluate the regulating effect of gastrodin (GAS) on striatal extracellular 5-hydroxytrypta‐ minen (5-HT) concentration in the rat models of tic disorders (TD) of children, and to explore the molecular mechanism. Methods Forty male Wistar rats were randomly divided into four groups: the control group, TD model group, GAS group and tiapride group. Except for the control group, in which rats were intraperitoneally injected with normal saline (0. 9%)(5 mL/kg), rats in the other three groups were injected with 3, 3’-iminodipropionitrile (IDPN)(150 mg/kg) for 7 days to develop the rats models of TD. After that, the related rats were treated respectively by oral gavage with normal saline at 10 mL/kg (the control group and the TD model group), GAS at 10 mg/kg (the GAS group), and tiapride at 1. 0 mg/kg (the tiapride group) once a day, for 8 weeks. Before and after the treatment, we studied the alterations in the stereotyped abnormalities of the rats on a weekly basis. The regulating effects of GAS on striatal extracellular 5-HT and 5-hydroxyindole acetic acid (5-HIAA) content were evaluated by intracerebral microdialysis and follow-up high-perfor‐ mance liquid chromatography (HPLC). The correlated changes in SERT protein expression was further determined by Western blotting after the rats were sacrificed and the striatum tissues were dissected. Results Compared with the con‐ trol group, the stereotyped movement of rats increased, the concentration of extracellular 5-HT decreased, and the gray value of SERT protein expression increased in the model group (all P<0. 05). Compared with the TD model group, the stereotyped movement of rats decrease in both the GAS group and the tiapride group after 4 weeks of intervention (both P< 0. 05); after 8 weeks of intervention, the striatal extracellular 5-HT concentration increased and SERT protein expression decreased in the GAS group (all P<0. 05), while the striatal extracellular 5-HT concentration and SERT protein expression did not change significantly in the tiapride group (all P>0. 05). There was no significant difference in the striatal extracellular 5-HT concentration between groups (P>0. 05). Conclusion Similar to tiapride, GAS could make a significant improvement on stereotyped behavioral abnormalities of TD rats, and the mechanism of action may be related to the regulation of 5-HT concentration and SERT protein expression. [ABSTRACT FROM AUTHOR]