A new heterometallic compound for the detection of acetylacetone and prevention activity on osteoarthritis.

A new inorganic-organic hybrid heterometallic compound, namely {[(CH3)2NH2][CdNa(4,4′-bpdc)2]}n·6n(H2O) (1, 4,4′-H2bpdc is 4,4′-biphenyldicarboxylic acid), was synthesized through the solvothermal self-assembly reactions of 4,4′-H2bpdc ligand in combination with Cd(II) and Na(I) salts. importantly, compound 1 shows high selectivity and sensitivity for the detection of acetylacetone (Hacac) molecule with a high Ksv value of 11.667 nM−1. Its prevention activity on osteoarthritis after the reconstruction of anterior cruciate ligament was investigated and the associated mechanism was analyzed. At first, the enzyme linked immunosorbent assay (ELISA) detection was implemented in the current research to detect the Interleukin-1β (IL-1β) and Interleukin-1β (IL-18) content released into joint cavity after treated with the compound. The results showed that this complex was good at inhibiting the IL-1β and IL-18 content released into joint cavity. The real time reverse transcription-polymerase chain reaction (RT-PCR) was carried out and the nuclear factor kappa-B (NF-κB) signaling pathway activation in joint soft tissue was tested exploiting real time RT-PCR. The real time RT-PCR exhibited that the NF-κB signaling pathway activation in the joint soft tissue was significantly reduced by the new compound. Subsequently, the levels of cartilage tissue cell apoptosis were also tested through Annexin V-FITC Apoptosis detection, and the results suggested the cartilage tissue cell apoptosis levels were obviously reduced by the compound. [ABSTRACT FROM AUTHOR]