Gdf15 对酒精性脂肪肝代谢异常的影响.

Objective: To investigate the effect of Growth and Differentiation Factor 15 ( GDF15) on alcoholic fatty liver (AFL). Methods: A model of mouse with alcoholic fatty liver disease was constructed with liquor, and then the control group and the model group were injected with AAV8-GDF15 molecules overexpressing the liver in the tail vein, and the mice were divided into four groups-normal + tail vein injection control AAV8-NC group (Con+AAV8-NC), model (alcohol) + tail vein injection AAV8 control virus group (AFL+AAV8-NC), normal + tail vein injection overexpressed AAV8-Gdf15 group (Con+ AAV8-Gdf15), alcohol + tail vein injection overexpressed AAV8-Gdf15 group (AFL+AAV8-Gdf15). By measuring body weight, fasting blood glucose, Intraperitoneal glucose tolerance test (IPGTT), insulin release test (ITT), and using test kits for their serum lipids, liver fats, alanine transaminase (ALT),Determination of aspartate transaminase (AST); liver biopsy sections were detected by hematoxylin-eosinstaining (HE) staining to detect structural abnormalities; The effect of over expression of GDF15 on alcoholic fatty liver was observed via q-PCR detecting of lipid metabolism-related molecular RNA levels, etc. Results: Compared with the Con+AAV8-NC group, weight loss was observed in group AFL+AAV8-NC. The weight loss in the AFL+AAV8-Gdf15 group was reduced compared to AFL+AAV8-NC after over expression of GDF15. Compared with the Con+AAV8-NC group, the fasting blood glucose in the AFL+AAV8-NC group increased, and the sugar tolerance and insulin resistance decreased. After overexpression of GDF15, the AFL+AAV8-Gdf15 group decreased significantly in fasting blood glucose and increased glucose tolerance and insulin resistance compared with AFL+AAV8-NC. The AFL+AAV8-NC group was significantly elevated compared with the Con+AAV8-NC group, and the lipids of the AFL+AAV8-Gdf15 group decreased significantly compared with those in the AFL+AAV8-NC group after overexpression of GDF15. Compared with the Con+AAV8-NC group, the liver weight of the AFL+AAV8-NC group increased, the degree of liver function damage was more severe, and the fat content of the liver increased. After the expression of GDF15, the liver weight, degree of damage and fat content of the liver were significantly reduced in group AFL+AV8-Gdf15 compared with AFL+AAV8-NC group. Conclusion: Alcoholic fatty liver disease increases the expression of GDF15, and increased expression of GDF15 will improve the damage and metabolic abnormalities of alcoholic fatty liver. [ABSTRACT FROM AUTHOR]