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Altered Expression of hsa_circ_0001445 and hsa_circ_0020397 in Breast Cancer Representing Associations with BMI and Reproductive Factors.
- Source :
-
Archives of Iranian Medicine (AIM) . Dec2022, Vol. 25 Issue 12, p817-827. 11p. - Publication Year :
- 2022
-
Abstract
- Background: Circular RNAs (circRNAs), one of the recent subclasses of non-coding RNAs (ncRNAs), show pivotal functions in regulation of gene expression and have significant roles in malignancies including breast cancer (BC). This study was aimed to assess the hsa_circ_0001445 and hsa_circ_0020397 expression and role in BC, as well as the potential circRNA/miRNA/mRNA crosstalk in these contexts. Methods: The expression of hsa_circ_0001445 and hsa_circ_0020397 in 50 breast tumors and 50 normal tissues adjacent to the tumors was investigated using quantitative real-time polymerase chain reaction (qRT-PCR). Finally, bioinformatics analyses were used to uncover hsa_circ_0001445, hsa_circ_0020397-miRNA-mRNA potential regulatory networks. Results: The hsa_circ_0001445 expression was considerably downregulated in malignant tissues compared to their normal counterparts (P = 0.020), while the hsa_circ_0020397 showed an upregulated pattern (P < 0.001). Additionally, it was observed that the higher expression of hsa_circ_0001445 was associated with hair dye avoidance (P = 0.034) and normal body mass index (BMI) (P = 0.016) while hsa_circ_0020397 over-expression had an important association with a lack of vitamin D consumption (P = 0.039). On the other hand, lower expression of hsa_circ_0001445 was significantly associated with age at menarche <14 years (P = 0.027). Our study also revealed that the two circRNAs have potential ability to regulate key mRNAs and miRNAs in competing endogenous RNA (ceRNA) networks. Conclusion: It is suggested that hsa_circ_0001445 and hsa_circ_0020397 with two opposite roles may be involved in BC development through sponging some miRNAs regulating ceRNA networks. However, their molecular interactions should be validated by further functional studies. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CIRCULAR RNA
*BIOCHEMISTRY
*PHENOMENOLOGICAL biology
*MICRORNA
*QUANTITATIVE research
*GENE expression
*BIOINFORMATICS
*COMPARATIVE studies
*GENES
*MESSENGER RNA
*DESCRIPTIVE statistics
*RESEARCH funding
*BODY mass index
*POLYMERASE chain reaction
*VITAMIN D deficiency
*BREAST tumors
*REPRODUCTIVE health
Subjects
Details
- Language :
- English
- ISSN :
- 10292977
- Volume :
- 25
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Archives of Iranian Medicine (AIM)
- Publication Type :
- Academic Journal
- Accession number :
- 163688666
- Full Text :
- https://doi.org/10.34172/aim.2022.127