刺五加华 E卫对去卵巢小鼠骨量及神经肽的影响.

Objective To investigate the effects of eleutheroside E (EE) on bone mass and neuropeptides in ovariectomized (OVX) mice. Methods Twenty-four 7-week-old C57BL6/ J female mice were randomly divided into Sham group (Sham), OVX group, EE low-dose group ( EE-L), and EE high-dose group ( EE-H). On the basis of OVX, mice in EE group received 20 mg/ (kg· d) of EE-Land 40 mg/ ( kg· d ) of EE-H by gavage. Mice in Sham and OYX groups were gavaged with an equal volume of 1 % carboxymethyl cellulose Na. After 12 weeks, the left femur and serum samples were collected. Micro-CT scan was performed on the left femur. The contents of TRAP, PlNP, TNF-a, IL-6, IL-10, CORP, SP, VIP, and NPY in serum were detected using ELISA. Results Compared with those in Sham, the levels of BMD, BV /TV, Tb.N, PlNP, IL-10, CORP, SP, VIP, and NPY decreased significantly in OVX group ( P<0. 05, P<0. 01), while Tb.Sp, TRAP, TNF-a and IL-6 increased significantly in OVX group ( P<0. 05). Compared with those in OVX, the levels of Tb.N, VIP and NPY in EE-L increased significantly ( P< 0. 05). BMD, BV /TV, IL- IO, CORP, and SP showed upward trend, but there was no significant difference ( P>0. 05). Tb. Sp, TRAP, TNF-a, and IL-6 showed a declined trend, but there was no significant difference ( P>0. 05). There was no change trend in Pl NP (P>0. 05). In EE-H group, BMD, BV/ TV, TB.N, PlNP, IL-10, CORP, SP, VIP, and NPY increased significantly (P<0. 05, P<0. 01), while Tb. Sp, TRAP, TNF-a, and IL-6 decreased significantly ( P < 0. 05). Conclusion EE significantly increases bone mass after OVX, possibly by reducing inflammation and increasing the content of neuropeptides in a dose-dependent manner. [ABSTRACT FROM AUTHOR]